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Preventing contamination and error
Onboarding Discussion Guide
Protocols
Resuspending primers
Extraction
QIAGEN DNeasy Plant Kit
QIAGEN DNeasy Tissue Kit
QIAGEN QIAamp Micro
DNA Extraction from formalin-fixed tissues
SPRI beads (hybrid)
TissueLyser II Homogenizer
DNA & sample cleanups
ExoSAP-IT PCR purification
Post-extraction insect sample cleaning
SPRI bead-based DNA cleanup
PCR
Bird sexing
Quantification
Gel Electrophoresis
NanoDrop
Plate Reader
Qubit
TapeStation
Library prep
NEBNext Ultra II FS DNA Library Prep Kit
NEBNext UltraExpress FS DNA Library Prep Kit
ONT Ligation sequencing amplicons - Native Barcoding Kit 96 V14
Sequencing
GENEWIZ Sanger sequencing protocol
Resources
PCR Recipes
Printing templates
Buffer conversion calculator
Library Pooling Guide
NUDIMAX
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Blue Loading Dye
Homemade SPRI Beads
Orange Loading Dye
ProK
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Lab Equipment
DNA shearing with the qSonica Sonicator
NanoDrop
TapeStation
TissueLyser II Homogenizer
Cal Academy Homepage
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Protocols
DNA & sample cleanups
DNA & sample cleanups
These protocols are primarily involved with DNA cleanup and purification.
Table of contents
ExoSAP-IT PCR purification
Post-extraction insect sample cleaning
SPRI bead-based DNA cleanup