PCR Recipes

Consult with CCG staff before using any resource with which you are unfamiliar. These resources are provided for convenience and reference only; ensure that you understand all calculations and workflows before implementing them into your project, and always independently verify formulas, calculations, and results.

PCR Recipe Calculator: A markdown file with annotations explaining an example PCR recipe using DreamTaq polymerase. Note that this example uses full doses of both betaine and BSA; this “kitchen sink” approach would be most appropriate for difficult samples.

PCR Recipes: A Google Drive folder with several PCR protocols commonly used in the CCG.